目的 联合溶剂提取和反溶剂沉淀一步制备纳米级的槐米总黄酮提取物。方法 分别以氯化胆碱-二缩三乙二醇低共熔体系(摩尔比1∶2,含水量20%)或体积分数65%乙醇作为溶剂提取槐米药材,所得提取液与水在高速剪切下混合得纳米混悬液,并进一步经冷冻干燥制得提取物粉末;考察并比较2种提取物的外观形态、粒径、溶解度和体外溶出度。结果 低共熔溶剂和乙醇提取液中总黄酮含量分别为(17.72±1.54)和(4.02±0.35) mg·g-1,相应的冻干粉中总黄酮含量分别为129.29 和20.29 mg·g-1,且混悬液及冻干粉复溶后粒径均小于500 nm;2种冻干粉中总黄酮溶解度均高于芦丁原料药,且在水中20 min时累积溶出即达85%以上,而芦丁2 h时溶出度不足60%。结论 该工艺简便有效,所得提取物溶解和溶出性能显著改善,且低共熔溶剂提取优于乙醇回流。
Abstract
OBJECTIVE To obtain nano-sized extract powder of total flavonoids from Flos Sophorae by combinational application of DES/ethanol extraction and anti-solvent precipitation. METHODS Flos Sophorae was extracted by ethanol or DES (choline chloride and triethylene glycol at molar ratio of 1∶2 containing 20% water). The extraction liquids were mixed with water to obtain suspensions, followed by freeze-drying to prepare extract powders. The extracts were compared on their appearance, particle size, saturated solubility and in vitro dissolution profiles in water. RESULTS Content of total flavonoids in extraction liquid was (17.72±1.54) mg·g-1 for DES and (4.02±0.35) mg·g-1 for ethanol, and that was 129.29 and 20.29 mg·g-1 for DES and ethanol extract powders, respectively. The extract powder of both DES and ethanol showed a larger solubility in water than rutin itself. And their dissolution rates reached over 85% at 20 min, whereas rutin released less than 60% at 2 h. CONCLUSION This method is simple and efficient, the obtained extract possesses favorable solubility and dissolution properties. DES shows a better performance than ethanol during the process.
关键词
低共熔溶剂 /
槐米 /
总黄酮 /
纳米混悬液 /
反溶剂沉淀 /
芦丁
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Key words
deep eutectic solvent /
Flos Sophorae /
total flavonoid /
nano-suspension /
anti-solvent precipitation /
rutin
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中图分类号：
R944
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参考文献
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脚注
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基金
国家自然科学基金项目资助(81760713);宁夏自然科学基金项目资助(2019AAC03233);宁夏回族自治区大学生创新创业训练计划项目资助(S201910752018)
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